Comparative proteomics: a tool for understanding drought tolerance in African grain crops.

–  Ndomelele Ndiko Ludidi –



Date of Birth: 02 June 1973
Citizenship: South African Home Address: 38 Sauvignon Street
Protea Heights BRACKENFELL, 7560
South Africa
Work Address: Department of Biotechnology, University of the Western Cape, Private Bag X17, Bellville, 7580, South Africa
Mobile/Cell Phone: +27 78 976 2376
Work Telephone: +27 21 959 2750 or
Languages: English (Good) and IsiXhosa (Good), Afrikaans (Poor)

Academic Qualifications

PhD: 2006, University of the Western Cape
MSc Cum Laude: 2001, University of the Western Cape
BSc Hons Cum Laude: 09/1999, University of the Western Cape BSc: 09/1998, University of the Western Cape
Senior Certificate: 01/1991, Makaula Senior S. School

Research Track Record

NRF Rating: C3
Two Best Citations: 142 Citations (Donaldson et al., 2004; FEBS Lett 569) 144 Citations (Ludidi & Gehring, 2003; J Biol Chem 278)
Publication Index: h-index = 11; i10 index = 12

Employment History

Position – Professor
Employer – University of the Western Cape
Duration – November 2015 to Current
Position – Deputy Dean (Research and Postgraduate Studies)
Employer – Faculty of Natural Sciences, University of the Western Cape
Duration – January 2018 to Current
Position – Chairperson (Department of Biotechnology)
Employer – University of the Western Cape
Duration – January 2015 to December 2017
Position – Assistant Dean (Research) : Faculty of Natural Sciences
Employer – University of the Western Cape
Duration – June 2014 to June 2015
Position – Deputy Chairperson (Department of Biotechnology)
Employer – University of the Western Cape
Duration – January 2013 to December 2014
Position – Associate Professor of Plant Biotechnology
Employer – University of the Western Cape
Duration – January 2011 to October 2015
Position – Senior Lecturer (Plant Biotechnology)
Employer – Stellenbosch University
Duration – 1 December 2010 to 31 December 2010
Position – Lecturer (Plant Biotechnology)
Employer – Stellenbosch University
Duration – February 2007 to December 2010
Position – Lecturer (Genetics and Cell & Molecular Biology)
Employer – University of the Witwatersrand
Duration – January 2006 to February 2007

Teaching Experience

Genetics, Biochemistry and Agricultural Biotechnology at 2nd year level of the 3 year BSc degree, together with Eukaryotic Gene Regulation, Biotechnology and Agricultural Biotechnology at the 3rd year level of the 3 year BSc degree and Advanced Plant Biotechnology at BSc Honours level.

Membership with professional bodies

Member of the South African Association of Botanists Member of the American Society of Plant Biologists
Member of the South African Society for Biochemistry and Molecular Biology

Research Visits

Visiting Researcher: University of Durham, United Kingdom (June 2016 to August 2016)
Visiting Researcher: University of Missouri, United States (March 2014 to June 2014)
Visiting Researcher: University of Durham, United Kingdom (June 2008 to August 2008)
Visiting Researcher: Rothamsted Research, United Kingdom (July 2006 to October 2006)

Journal Articles

1. Phillips K, Ludidi N. Drought and exogenous abscisic acid alter hydrogen peroxide accumulation and differentially regulate the expression of two maize RD22-like gene. Sci Rep. 2017; 7:8821.
2. Ullah S, Kolo Z, Egbichi I, Keyster M, Ludidi N. Nitric oxide influences glycine betaine content and ascorbate peroxidase activity in maize. S Afri J Bot. 2016; 105:218-225.
3. Klein A, Keyster M, Ludidi N. Response of soybean nodules to exogenously applied caffeic acid during NaCl-induced salinity. S Afr J Bot. 2015; 96:13-18.
4. Simon-Sarkadi L, Ludidi N, Kocsy G. Modification of cadaverine content by NO in salt- stressed maize. Plant Signal Behav. 2014; 8: e27598.
5. Egbichi I, Keyster M, Ludidi N. Effect of exogenous application of nitric oxide on salt stress responses of soybean. S Afr J Bot. 2014;90:131-136.
6. Egbichi I, Keyster M, Jacobs A, Klein A, Ludidi N. Modulation of antioxidant enzyme activities and metabolites ratios by nitric oxide in short-term salt stressed soybean root nodules. S Afr J Bot. 2013;88:326-333.
7. Klein A, Keyster M, Ludidi N. Caffeic acid decreases salinity-induced root nodule superoxide radical accumulation and limits salinity-induced biomass reduction in soybean. Acta Physiol Plant. 2013;35:3059-3066.
8. Boldizsár A, Simon-Sarkadi L, Szirtes K, Soltész A, Szalai G, Keyster M, Ludidi N, Galiba G, Kocsy G. Nitric oxide affects salt-induced changes in free amino acid levels in maize. J Plant Physiol. 2013;170:1020-1027.
9. Keyster M, Adams R, Klein A, Ludidi N. Nitric oxide (NO) regulates the expression of single-domain cystatins in Glycine max (soybean). Plant Omics. 2013;6:183-192.
10. Keyster M, Klein A, Du Plessis M, Jacobs A, Kappo A, Kocsy G, Galiba G, Ludidi N. Capacity to control oxidative stress-induced caspase-like activity determines the level of tolerance to salt stress in two contrasting maize genotypes. Acta Physiol Plant. 2013; 35:31-40.
Journal Articles (continued)
11. Keyster M, Klein A, Ludidi N. Caspase-like enzymatic activity and the ascorbate- glutathione cycle participate in salt stress tolerance of maize conferred by exogenously applied nitric oxide. Plant Signal Behav. 2012;7:349-360.
12. Mulaudzi T, Ludidi N, Ruzvidzo O, Morse M, Hendricks N, Iwuoha E, Gehring C. Identification of a novel Arabidopsis thaliana nitric oxide-binding molecule with guanylate cyclase activity in vitro. FEBS Lett. 2011;585:2693-2697.
13. Keyster M, Klein A, Egbich I, Jacobs A, Ludidi N. Nitric oxide increases the enzymatic activity of three ascorbate peroxidase isoforms in soybean root nodules. Plant Signal Behav. 2011;6:956-961.
14. Keyster M, Klein A, Ludidi N. Endogenous NO levels regulate nodule functioning: potential role of cGMP in nodule functioning? Plant Signal Behav. 2010;5:1679-81.
15. Leach J, Keyster M, Du Plessis M, Ludidi N. Nitric oxide synthase activity is required for development of functional nodules in soybean. J Plant Physiol. 2010;167:1584-91.
16. Bastian R, Dawe A, Meier S, Ludidi N, Bajic VB, Gehring C. Gibberellic acid and cGMP-dependent transcriptional regulation in Arabidopsis thaliana. Plant Signal Behav. 2010;5:224-32.
17. Ludidi NN, Pellny TK, Kiddle G, Dutilleul C, Groten K, VAN Heerden PD, Dutt S, Powers SJ, Römer P, Foyer CH. Genetic variation in pea (Pisum sativum L.) demonstrates the importance of root but not shoot C/N ratios in the control of plant morphology and reveals a unique relationship between shoot length and nodulation intensity. Plant Cell Environ. 2007;30:1256-68.
18. Ludidi N, Morse M, Sayed M, Wherrett T, Shabala S, Gehring C. A recombinant plant natriuretic peptide causes rapid and spatially differentiated K+, Na+ and H+ flux changes in Arabidopsis thaliana roots. Plant Cell Physiol. 2004;45:1093-8.
19. Donaldson L, Ludidi N, Knight MR, Gehring C, Denby K. Salt and osmotic stress cause rapid increases in Arabidopsis thaliana cGMP levels. FEBS Lett. 2004;569:317-20.
20. Ludidi N, Gehring C. Identification of a novel protein with guanylyl cyclase activity in
Arabidopsis thaliana. J Biol Chem. 2003;278:6490-4.
21. Ludidi NN, Heazlewood JL, Seoighe C, Irving HR, Gehring CA. Expansin-like molecules: novel functions derived from common domains. J Mol Evol. 2002;54:587- 94.

Chapters in Books

1. Ludidi N. Measurement of nitric oxide in plant tissue using difluorofluorescein and oxyhemoglobin. In: Cyclic Nucleotide Signaling in Plants. Methods Mol Biol. 2013;1016:253-259. (ISBN ISBN 978-1-62703-441-8; ISSN: 1064-3745)

Activities outside Main Employment

1. Academic Journal Peer-review Panels and Editorial Board Membership
i. Associate Editor for Journal of Cell and Plant Sciences
ii. Editorial Board member of Journal of Plant Molecular Biology and Biotechnology
iii. Regular reviewer of manuscripts submitted for publications in several international academic journals
2. Research Funding Applications Panels
Reviewer and Panel Member for several applications submitted for funding to the National Research Foundation (NRF).
3. External Examination
I regularly act as examiner of MSc and PhD theses for various national (University of the Witwatersrand, University of Cape Town, University of Fort Hare and Stellenbosch University) and international (Monash University in Melbourne, Australia) universities. I also act as moderator for undergraduate examinations at the Cape Penninsula University of Technology and at Stellenbosch University.
4.Legislative Panels
Member of the South African National Department of Agriculture’s GMO AdvisoryCommittee of the Directorate of Biosafety responsible for reviewing applications (made byacademic and industrial organizations) affected by the Genetically Modified Organisms Act(Act No.15 of 1997).

Relevant Professional Skills

Teaching at undergraduate level, acquisition and management of research funds, administrative and managerial experience at departmental and faculty level within an academic institution, management of large research projects, supervision of staff and postgraduate students, national and international collaboration in research.

Current Research Interests

I am interested in the characterization of the molecular mechanisms mediating plant responses to drought and salinity stress. My aim is to develop new crop varieties with enhanced tolerance to these stresses in order to improve food security.